RENOPROTECTIVE AND HEPATOPROTECTECTIVE POTENTIALS OF L-ARGININE AND GLUTAMATE ON STREPTOZOTOCIN INDUCED DIABETIC ALBINO RATS.
DOI:
https://doi.org/10.61841/cxv6w744Abstract
Diabetes mellitus is one of the most common metabolic disorders globally. L-arginine and glutamate have promising beneficial effects against metabolic disorders. Renoprotective and Hepatoprotectective potentials of L-Arginine and Glutamate on Streptozotocin Induced Diabetic albino rats. A total of 73 adult albino rats were used. Out of this, 13 rats were used for LD50 study while 60 rats were used for the proper study. They were grouped into 6, Group 1 served as Normal control, Group 2-6 were diabetic rats induced with streptozotocin (120mg/kg bwt) served as Diabetic control (administered only 0.2 ml normal saline daily). Groups 3, 4, 5 and 6 rats were diabetic rats given Glibenclamide (3 mg/kg bwt), L-Arginine (50 mg/kg bwt), Glutamate (50 mg/kg bwt), L-Arginine and Glutamate (50 mg/kg bwt), respectively for 30 days. After the treatment period, the animals were sacrificed; blood sample collected, and serum separated for determination of liver function status, lipid profile, kidney function markers, There were significant (P<0.05) decrease in the activities of AST and ALT when the treatment groups were compared to the diabetic group, the L-arginine group showed the lowest AST activity followed by the Glibenclamide control group then the group co-treated with L-arginine and glutamate. The effects of L-arginine and glutamate on renal function; urea, creatinine showed a significant decrease (p<0.05) in the l-arginine and glutamate treated group when compared to the diabetic group, and in sodium, potassium and chloride levels when compared to the drug control group. It could be concluded that L-arginine and glutamate have also showed reno-protective and hepato-protective potentials.
References
Abdel, N and Hamed, M. (2016). Alterations in hematological Parameters: could it be a marker in diabetes mellitus? British medical journal of Diabetic. ;2(1):1–9.
Adebayo, S, A., Ondua, M., Shai, L. J. and Lebelo S. L. (2019). Inhibition of nitric oxide production and free radical scavenging activities of four South African medicinal plants. Journal of Inflammation Research.;12:195-203.
Charen, E. and Harbord, N. (2020). Toxicity of Herbs, Vitamins, and Supplements. Advances in chronic kidney disease, 27 (1), 67–71.
Dabbou, S., Gai, F. and Renna, M.(2017). Inclusion of bilberry pomace in rabbit diets: Effects on carcass characteristics and meat quality. Metabolism Science.;124:77–83.
Darenskaya, M. A., Kolesnikova, L. I. and Kolesnikov S. I. (2021). Oxidative Stress: Pathogenetic Role in Diabetes Mellitus and Its Complications and Therapeutic Approaches to Correction. Bulletin of Experimental Biology and Medicine 171:179–189.
Demirtas, L., Degirmenci, H., Akbas, E. M., Ozcicek, A., Timuroglu, A. and Gurel A. (2015). Association of hematological indicies with diabetes, impaired glucose regulation and microvascular complications of diabetes. International Journal of Clinical and Experimental medicine; 8(7):11420–1427.
Dennis, J. and Witting, P. (2017). Protective Role for Antioxidants in Acute Kidney Disease. Nutrients. 9. 10.3390/9070718.
Dmour, H. H.,Khreisat, E. F., Khreisat, A. F., Hasan, S. A., Atoom, O. and Alkhatib, A. J. (2020). Assessment of Lactate Dehydrogenase Levels Among Diabetic Patients Treated in the Outpatient Clinics at King Hussein Medical Center, Royal Medical Services, Jordan. Medical archives (Sarajevo, Bosnia and Herzegovina), 74 (5), 384–386.
Egbuonu, A.C.C., Opara, C.I., Akachukwu, D. and Onyedikachi, U.B. (2018). Effect of ethanolic extract of avocado pear (Persea americana) seed on normal and monosodium glutamate-compromised rats' hepatic histo-morphology and serum bio-functional parameters. Research Journal of Environmental Science, 12: 53-62.
Matsuda A, Kuzuya T, Sugita Y, Kawashima K. (2014) Plasma levels of glib¬enclamide in diabetic patients during routine clinical administration determined by specific radioimmunoassay. Horm Metab Res. 15: 425–428.
Miyazawa, N., Yoshimoto, H., Kurihara, S., Hamaya, T. and Eguchi, F. (2018). Improvement of Diet-induced Obesity by Ingestion of Mushroom Chitosan Prepared from Flammulina velutipes. J. Oleo Science, 67, 245–254.
Modawe, G., Mohammed, I., Dafalla, A. and Mohieldein, A. (2023) Evaluation of Plasma Malondialdehyde among Sudanese Type 2 Diabetic Patients. Open Journal of Endocrine and Metabolic Diseases, 13, 234-243.
Moghadam-Kia, S., Oddis, C. V, and Aggarwal, R. (2016). Approach to asymptomatic creatine kinase elevation. Cleve Clinical Journal of Medicine. 83 (1):37-42.
Nahid Mh, Elamin, Fadlalla Imt, Omer Shadia A, And Ibrahim Hala Am. (2018) “Histopathological Alteration In Stz-Nicotinamide Diabetic Rats, A Complication Of Diabetes Or A Toxicity Of Stz?” International Journal Of Diabetes And Clinical Research 5, No. 3..
Nakai, K., Umehara, M., Minamida, A. (2023) Streptozotocin induces renal proximal tubular injury through p53 signaling activation. Scientific Report 13, 8705
Obidike, I. J and Chita, E.I.(2020). Nutraceutical, antioxidant and hepatic histomorphological effects of Tetrapleura tetraptera leaves in monosodium glutamate-intoxicated rats. Asian J. Emerging Res.,2: 223-238.
Obidike, I. J and Mbah, E. S (2024). Effects of Carrot (Daucus carota) Stalk on Blood Glucose Level, Biochemical Functions and Serum Antioxidant Activity in Alloxan-Induced Diabetic Rats. Asian Scientific Bulletin. 2 (1): 46-59.
Obidike, I. J., Aloh, G. S., Obike, C. A. and Okechukwu, J. N. (2024). Evaluation of Anti-Ulcerogenic Effects of the Crude Extract and Fractions of Dialium guineense Tree Bark in Ethanol-Induced Peptic Ulcer in Albino Rats. Asian Journal of Biological Science, 17 (3): 254-273
Obidike, I. J., Chita, E. I., Nankwo, C. I and Owoh, Q. C.(2023). Effects of Tetrapleura tetraptera leaves on renal architecture and haematological indices in monosodium glutamate-intoxicated rats. Asian Scientific Bulletin, 1: 8-17.
Obidike, I. J., Nwankwo I. C, and Ossai P. O. (2022). Consequence of Tetrapleura tetraptera Leaves on Pro-Oxidants, Hepatic Functions and Histomorphology in Monosodium Glutamate-Intoxicated Rats. Research Journal of Medicinal Plants. 16 (2): 37-48,
Obidike, I.J.and Egbuonu, A.C. (2019). Effects of ethanol extract of cocoa (theobroma cacao) pod on normal and monosodium glutamate-intoxicated rats' hepatic histo-morphology, serum bio-functional parameters and serum antioxidant activities. International Journal of Recent Research and Applied Studies. 6, 11(1), 1-13.
Obidike,I.J. and Egbuonu, A.C.(2020). Some Biochemical Effect of Cocoa (Theobroma Cacao) Pod Ethanol Extract on Renal Histo-Morphology and Function in Monosodium Glutamate (MSG)- Intoxicated Rats. International Journal of Recent Research and Applied Studies, 5(3), 17-28.
Ochei,J. and Kolhatkar, A (2008) Medical Laboratory Science, Theory and Practices. Tata McGraw-Hill, New York, 311-347.
Oguntibeju, O. O. (2019). Type 2 diabetes mellitus, oxidative stress and inflammation: Examining the links. Int. J. Physiol. Pathophysiol. Pharmacol.;11:45
Patikorn, C., Roubal, K., Veettil, S. K., Chandran, V., Pham, T., Lee, Y. Y., Giovannucci, E. L., Varady, K. A. and Chaiyakunapruk, N.(2021). Intermittent Fasting and Obesity-Related Health Outcomes: An Umbrella Review of Meta-analyses of Randomized Clinical Trials. JAMA Netw Open. 01; 4(12):213-219.
Pautz, A., Li, H. and Kleinert, H.(2021). Regulation of NOS expression in vascular diseases. Front. Biosci.;26:85–101.
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